FIG. 1.

Targeted disruption of mouse Zpr1. (A) Exon-intron structure (coding and noncoding exons are numbered and represented by solid and open boxes, respectively) and restriction map of the mouse Zpr1 gene, structure of the targeting vector, and structure of the mutated allele after homologous recombination. (B) The mutated Zpr1 allele was detected by Southern blot analysis and PCR. Genomic DNA from targeted embryonic stem cells was digested with EcoRI and detected with cDNA probe (exons 10 to 14; bp 954 to 1379). The 17.5-kb fragment corresponding to the wild-type (WT) allele and the 9.0-kb fragment corresponding to the mutated (knockout [KO]) allele are indicated. (C) Genomic DNA samples were also examined by PCR; the wild-type (WT) allele (250 bp) and mutated (KO) allele (444 bp) are shown. (D) Genomic DNA prepared from E3.5 embryos cultured in vitro for 96 h was genotyped by PCR.