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. 2005 Apr;25(7):2744–2756. doi: 10.1128/MCB.25.7.2744-2756.2005

FIG. 3.

FIG. 3.

ZPR1 deficiency results in defective embryo growth and causes mislocalization of SMN and coilin. (A) Blastocysts (E3.5) cultured in vitro were labeled with 50 μM BrdU for 1 h at 37°C. Wild-type (WT) and mutant (KO) embryos were stained with mouse monoclonal antibodies to ZPR1 (red), SMN (purple), and BrdU (green) and examined by indirect-immunofluorescence microscopy. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. (B) Colocalization of ZPR1 and coilin in mouse embryos. Blastocysts were stained with antibodies to coilin (red) and ZPR1 (green). Colocalization of coilin (red) and ZPR1 (green) is represented by yellow. Nuclei were stained with DAPI (blue). Scale bar, 8.0 μm. (C) Colocalization of SMN and ZPR1 in mouse embryos. Blastocysts (E3.5) cultured for 96 h were stained with antibodies to SMN (red) and ZPR1 (green) and examined by confocal laser-scanning immunofluorescence microscopy. Colocalization of SMN (red) and ZPR1 (green) is represented by yellow. Scale bar, 8.0 μm.