FIG. 4.

ZPR1 deficiency causes defects in snRNP localization. (A) Loss of ZPR1 results in nuclear and nucleolar accumulation of Sm proteins. Wild-type (WT) and mutant (KO) blastocysts (E3.5) were cultured in vitro for 96 h and stained with antibodies to Sm proteins (green). All stained samples were examined by confocal laser-scanning immunofluorescence microscopy. Nuclei were stained with DAPI (blue). Scale bar, 20 μm. (B) Deficiency of ZPR1 causes nuclear and nucleolar accumulation of snRNPs. Embryos were stained with antibodies to TMG 5′-cap of U snRNA for detection of snRNPs (red) in wild-type and mutant embryos. Scale bar, 20 μm.