FIG. 6.

Deficiency of ZPR1 causes defects in axons of differentiated NSC-34 cells. (A) Loss of ZPR1 causes axonal defects in differentiated NSC-34 cells that resemble motor neurons. Cells transfected with scrambled siRNA (Control) and ZPR1-specific siRNA (siRNA) were cultured for 72 h and stained with antibodies to tubulin (red) and ZPR1 (green). The arrowhead indicates the kinks in the axon of motor neuron after knockdown of ZPR1 (siRNA). Scale bar, 20 μm. (B) ZPR1 deficiency results in the loss of growth cones of differentiated NSC-34 cells. ZPR1 was stained with antibodies to ZPR1 (green), and actin was stained with phalloidin conjugated with Alexa 546 (red). Nuclei were stained with DAPI (blue). All samples were examined by confocal laser-scanning fluorescence microscopy. Scale bar, 20 μm.