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. 2005 Apr;25(7):2733–2743. doi: 10.1128/MCB.25.7.2733-2743.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 10. — JIP4 requires MKK3 and MKK6 for the potentiation of p38 MAP kinase activation. (A) Total protein extracts were prepared from wild-type MEF, Mkk3⁻^/⁻ MEF, Mkk6⁻^/⁻ MEF, and Mkk3⁻^/⁻ Mkk6⁻^/⁻ compound mutant MEF. The expression levels of MKK3, MKK6, and tubulin were examined by immunoblot analysis. (B) The effects of JIP3 and JIP4 on ASK1-stimulated p38 MAPK activity in wild-type MEF were examined by cotransfection assays. The activity of HA-tagged p38α MAP kinase was examined in an immune complex kinase assay using the substrate ATF2. (C) The effects of JIP3 and JIP4 on ASK1-stimulated p38α MAPK activity in an Mkk3⁻^/⁻ Mkk6⁻^/⁻ compound mutant MEF were examined by cotransfection assays. The activity of HA-tagged p38α MAP kinase was examined in an immune complex kinase assay using the substrate ATF2.