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JIP4 forms homo-oligomers but does not interact with other JIP proteins. The interactions between JIP proteins were examined. Flag-tagged JIP3 and JIP4 proteins were coexpressed with T7-tagged JIP3 or JIP4 (A) and JIP1 or JIP2 (B). The JIP3 and JIP4 proteins were immunoprecipitated (IP) with the Flag (M2) or T7 antibody. The coimmunoprecipitation of other JIP proteins was examined by immunoblot (IB) analysis. JIP3/4, JIP3 and JIP4; JIP1/2, JIP1 and JIP2.
