Skip to main content
. 2005 Apr;25(7):2607–2621. doi: 10.1128/MCB.25.7.2607-2621.2005

FIG. 9.

FIG. 9.

In vitro reconstitution showing JAK2 activation and inhibition by SH2-B. Recombinant JAK2 and recombinant SH2-B (indicated concentrations) were incubated with ATP (1.2 mM). Experiments were conducted with JAK2 at (A and B) 14 pM or (C and D) 118 pM concentrations. In panels A and C, JAK2 was visualized immunoblots with anti-pY and anti-JAK2 antibodies; in panels B and D, the results from three separate experiments were quantified by scanning densitometry and combined (mean ± the standard error of the mean; ✽, P < 0.05).