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. 2005 Apr;25(7):2622–2631. doi: 10.1128/MCB.25.7.2622-2631.2005

FIG. 2.

FIG. 2.

Embryos generated from Gata6−/− ES cells by tetraploid embryo complementation complete gastrulation. (A) Micrographs of E9.5 and E10.5 embryos generated from either Gata6+/− or Gata6−/− ES cells. (B) Schematic showing position of primers relative to the Gata6 wild-type allele and null allele in which exons encoding the zinc finger domains of GATA6 were replaced by Neo; note that one of the Gata6 oligonucleotides contained sequences encoding a portion of the first zinc finger that were deleted in Gata6−/− ES cells. wt, wild type; mut, mutant. (C) PCR analysis with primers that amplify Gata6 genomic DNA or the Neo gene confirms that Gata6 was present in control Gata6+/+ and Gata6+/− ES cells and embryos (lanes 2 to 5) but was absent from embryos derived from Gata6−/− ES cells (lanes 6 to 10) or in reaction mixtures lacking template (0 DNA, lane 1).