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. 2023 Oct 30;7(1):e202302222. doi: 10.26508/lsa.202302222

Figure S3. Cytokine analysis in BMDM supernatants.

Figure S3.

Analysis of cytokine/chemokine protein secretion into the medium of BMDM. BMDM from male 9-wk-old controls, M-TTP KO, and M-Triple KO mice were cultured and treated with 1 μg/ml LPS, and cell culture supernatants were collected after various time points of LPS treatment. (A, B, C, D, E, F) Levels of (A) TNF, (B) KC (CXCL1), (C) IL-10, (D) MIP-2 (CXCL2), (E) IL-1β, and (F) MIG (CXCL9) in the macrophage supernatant were determined by a plate-based immunoassay by EVE Technologies. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; using two-way ANOVA with Šídák’s multiple comparison test.