Estimated by regression analyses. The association between testosterone and BMD z scores in individuals assigned male at birth was not assessed because testosterone was low (range, 0.5-1.1 nmol/L) in all individuals assigned male at birth at follow-up. Squares, triangles, and circles represent regression coefficients (β); whiskers represent 95% CIs. SI conversion factors: To convert estradiol from picomoles per liter to picograms per milliliter, divide by 3.671; to convert LH from international units per liter to milli-International units per milliliter, divide by 1.0; to convert testosterone from nanomoles per liter to nanograms per deciliter, divide by 0.0347; to convert 25-hydroxyvitamin D from nanomoles per liter to nanograms per milliliter, divide by 2.496. BMI is calculated as weight in kilograms divided by height in meters squared. LH indicates luteinizing hormone.