TABLE 2.

PCRs and thermal-cycling parameters for amplification of Cryptosporidium DNA

PCR method (reference)	MgCl2 concn (mM)	Amt of Taq (U)	Cycle conditions					No. of cycles	Restriction site(s)
			Initial denaturation	Denaturation	Annealing	Extension	Final extension
Laxer (15)	2	1	95°C, 10 min	94°C, 1 min	56°C, 90 s	72°C, 90 s	72°C, 7 min	45
Laberge (14)	2	1	95°C, 10 min	94°C, 1 min	56°C, 90 s	72°C, 90 s	72°C, 7 min	45
Wagner-Wiening (28)	2	2	94°C, 5 min	94°C, 1 min	50°C, 1 min	72°C, 1 min	72°C, 10 min	35	BamHI
Morgan (18)	2	1	95°C, 10 min	94°C, 1 min	58°C, 90 s	72°C, 90 s	72°C, 10 min	45
Johnson (13)	4	1	94°C, 5 min	94°C, 30 s	55°C, 30 s	72°C, 1 min	72°C, 10 min	40
Awad-El-Kariem (1)	2	2	94°C, 5 min	94°C, 90 s	47°C, 90 s	72°C, 3 min	72°C, 10 min	45	MaeI
Rochelle (24)	2	1	94°C, 2 min	94°C, 30 s	55°C, 30 s	72°C, 90 s	72°C, 5 min	40
Bonnin (3)	2.5	1.5	94°C, 20 min	94°C, 90 s	45°C, 90 s	72°C, 90 s	72°C, 10 min	45	HinfI, RsaI