FIG. 4.

V-ATPase modulation does not alter apoptotic regulation of regrowth. (A) Quantification of apoptotic cell numbers during regrowth using the activated Caspase-3 antibody. P > 0.05 (n = ≥9 per timepoint). Data are means ± SEM. (B) Experimental strategy for rescue of the apoptotic inhibition phenotype by expression of the yeast H⁺ pump, PMA1.2. (C) Representative brightfield images of the outcomes from (B). Open arrowheads indicate regrowth-incompetent eyes. Scale bar = 500 μm. (D) Eyes were scored for the RI and quantified for regrowth quality (n = 47 M50054, n = 58 PMA injection+M50054). SEM, standard error of the mean.