FIG. 5.

Increased H⁺ pump activity restored eye regrowth. (A) Eyes were scored for the RI and quantified for regrowth quality. The data show that PMA1.2 expression is sufficient to rescue eye regrowth during V-ATPase inhibition. (B) Measurements of 5 dps eye size show PMA1.2 expression restored the V-ATPase−inhibited small eye phenotype (n = 22) to normal as compared with regrowth-incompetent eyes treated with Concanamycin A only (n = 12). **P < 0.01. Data are means ± SD. (C) Restoration of eye size by PMA1.2 expression (yellow arrowhead; n = 30). (D) An eye section showing membrane expression of PMA1.2 (green). Nuclei are shown by DAPI (red). n = 5. Scale bar = 50 μm.