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. 1989 May;90(1):251–258. doi: 10.1104/pp.90.1.251

Changes in Sugars, Enzymic Activities and Acid Phosphatase Isoenzyme Profiles of Bananas Ripened in Air or Stored in 2.5% O2 with and without Ethylene 1

Angelos K Kanellis 1,2,2, Theophanes Solomos 1,2, Autar K Mattoo 1,2
PMCID: PMC1061706  PMID: 16666745

Abstract

This study investigates the effect of 2.5% O2, both alone and in combination with ethylene, on respiration, sugar accumulation and activities of pectin methylesterase and acid phosphatase during ripening of bananas (Musa paradisiaca sapientum). In addition, the changes in the phosphatase isoenzyme profiles are also analyzed. Low oxygen diminished respiration and slowed down the accumulation of sugars and development of the yellow color. Furthermore, low O2 prevented the rise in acid phosphatase activities and this suppression was not reversed by the inclusion of 100 microliters per liter ethylene in 2.5% O2 atmosphere. Gel electrophoresis of both the soluble and particulate cell-free fractions under nondenaturing conditions revealed the presence of 8 and 9 isoenzymes in the soluble and particulate fractions, respectively. Low O2 suppressed the appearance of all isoenzymes, and the addition of 500 microliters per liter ethylene to the low oxygen atmosphere did not reverse this effect. Similarly, the decline in pectin methylesterase that was observed in air-ripened fruits was prevented by 2.5% O2 alone and in combination with 500 microliters per liter ethylene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Awad M., Young R. E. Postharvest Variation in Cellulase, Polygalacturonase, and Pectinmethylesterase in Avocado (Persea americana Mill, cv. Fuerte) Fruits in Relation to Respiration and Ethylene Production. Plant Physiol. 1979 Aug;64(2):306–308. doi: 10.1104/pp.64.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett A. B., Smith G. M., Nichols B. G. Regulation of climacteric respiration in ripening avocado fruit. Plant Physiol. 1987 Apr;83(4):973–976. doi: 10.1104/pp.83.4.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burg S. P., Burg E. A. Molecular requirements for the biological activity of ethylene. Plant Physiol. 1967 Jan;42(1):144–152. doi: 10.1104/pp.42.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burg S. P. Ethylene in plant growth. Proc Natl Acad Sci U S A. 1973 Feb;70(2):591–597. doi: 10.1073/pnas.70.2.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Leo P., Sacher J. A. Senescense: association of synthesis of Acid phosphatase with banana ripening. Plant Physiol. 1970 Aug;46(2):208–211. doi: 10.1104/pp.46.2.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Marriott J. Bananas--physiology and biochemistry of storage and ripening for optimum quality. Crit Rev Food Sci Nutr. 1980;13(1):41–88. doi: 10.1080/10408398009527284. [DOI] [PubMed] [Google Scholar]
  8. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  9. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Young R. E., Biale J. B. Phosphorylation in avocado fruit slices in relation to the respiratory climacteric. Plant Physiol. 1967 Oct;42(10):1357–1362. doi: 10.1104/pp.42.10.1357. [DOI] [PMC free article] [PubMed] [Google Scholar]

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