TABLE 2.

Purification of PAD from recombinant E. coli^a

Purification step	Total protein (mg)	Total activity (U)b	Sp act (U mg−1)	Purifi- cation (fold)	Yield (%)
Crude extract	350	875	2.5	1	100
(NH4)2SO4 (saturation)	85	637	7.5	3	73
Q-Sepharose (pH 7)	21	504	24	9.6	58
Methyl HIC (pH 7)	3.3	297	90	36	34
DEAE-Sepharose (pH 7)	0.6	123	205	82	14
Methyl HIC (pH 7)	0.25	70	280	112	8

^aFor details see the text. 

^bOne unit corresponds to 1 μmol of ferulic acid decarboxylated per min.