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Schema of 8-week and 12-week animal exposures. Mice were exposed to aerosols from 3 products for 8 weeks (A) or 12 weeks (B). Air-exposed animals served as control for each group. Mice exposed for 12 weeks were vaccinated i.m. at wk5, wk6 and wk7 after the start of exposures, and were given acute intratracheal challenge with NTHI (106 cfus/mouse in 50uL PBS) at the end of the wk12 for 0, 4 and 12 h
