Fig. 6.

Chronic inhalatory exposures to EC, HTP and CC aerosols induce lung damage. Mice exposed to aerosols from alternative tobacco products and CC for 8 weeks were euthanized and BAL harvested. MPO activity, levels of NE and antioxidant potential in the BAL were measured as described previously [47] and details provided in Additional file 1. Data are presented as bar diagrams with mean ± SE. Difference between two groups is considered significant at p < 0.05 and is indicated by symbols *p < 0.05, **p < 0.01, ***p < 0.001 ****p < 0.0001, calculated after performing a non-parametric Kruskal–Wallis rank test with FDR correction for multiple comparisons by employing GraphPad Prism V.9 software (GraphPad; La Jolla, California, USA). We used n = 10 animals for air (5 M + 5F) and n = 20 animals for EC, HTP, and CC (10M + 10F) per group for each exposure condition