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. 2023 Oct 31;27:107. doi: 10.1186/s40824-023-00446-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Assessment of biological activity of AEC patch in vitro. (A) Schematic of AEC patch-contained secretomes transport to the cells using a transwell setting. (B) Gross observation of hSFB scratch wound closure with time. The yellow line indicates the uncovered wound area with the cells. Scale bar is 500 μm. (C) Quantification of the wound closure rate at different time points for 24 h. (D) Cell proliferation of hSFB when treated with each patch group as measured by CCK-8 assay for up to 10 days. (E) Immunofluorescence staining of collagen type I (green) as synthesized by hSFB, along with DAPI staining (blue). (F) Quantification of collagen I positive area. (G) Immunofluorescence staining of HUVEC (blue: DAPI, green: CD31, red: F-actin). (H) Quantitative analysis of positively stained area of F-actin. Statistically significant difference (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)