Fig 5.

The combined boosting broadened primary T-cell responses. (A) Scheme of experiments. The immunization schemes were identical to Fig. 4. Lung tissues were harvested at 14 days post-last vaccination. (B–E) Frequencies of pulmonary CD8⁺ T cells (B and C) and CD4⁺ T cells (D and E) producing cytokines following re-stimulation with peptide pools for S1 (B and D) and TCEs (C and E). (F) Scheme of experiments. The immunization schemes were identical to Fig. 4. Spleens were harvested at 14 days post-last vaccination. (G–J) Frequencies of splenic CD8⁺ T cells (G and H) and CD4⁺ T cells (I and J) producing cytokines following re-stimulation with peptide pools for S1 (G and I) and TCEs (H and J). All data are represented as mean ± SEM and analyzed by one-way ANOVA with Tukey correction. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001.