Fig 4.

EDIM is enhanced by most defensins. EDIM was incubated with the indicated concentrations of (A) RED1, (B) RED3, (C) RMAD1, (D) RMAD4, (E) HD5, (F) HNP1, or (G) Crp2 before infecting MA104 cells (protocol 1; solid, colored lines). For protocol 2 (dotted, black lines with filled circles), EDIM was bound to cells in the cold prior to defensins being added (A, B, and D–G). Data are normalized to infection in the absence of defensin (control infection) and are the mean ± SD of at least three individual experiments.