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. 2023 Oct 13;97(10):e00426-23. doi: 10.1128/jvi.00426-23

TABLE 2.

Plasmid list

Plasmid name Backbone Epitope tag Selection
pCAG.MCS2 pCAG.MCS2 Amp
pCAG.Myc pCAG.Myc Amp
pCAG.MCS2-Myc pCAG.MCS2-Myc Amp
pcDNA3.1 MycHis(−)A pcDNA3.1 MycHis(−)A Myc Amp
pEGFP-C1 pEGFP-C1 EGFP Kan
pCAG.MCS2-FLAG-HiBiT pCAG.MCS2-FLAG-HiBiT FLAG, HiBiT Amp
pCAG.HiBiT-FLAG pCAG.HiBiT-FLAG-MCS2 FLAG, HiBiT Amp
pCAG.SARS-CoV-2-S-FLAG-HiBiT pCAG.MCS2-FLAG-HiBiT FLAG, HiBiT Amp
pCAG.SARS-CoV-2-M pCAG.MCS2 Amp
pCAG.SARS-CoV-2-N pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E pCAG.MCS2 Amp
pCAG.SARS-CoV-2-ORF3A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-F20/F23/F26A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-C40/C43A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-F56/Y57/Y59A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-N66A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-D72/L73/L74/V75A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-K63N pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-N15A pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-V25F pCAG.MCS2 Amp
pCAG.SARS-CoV-2-E-T9I pCAG.MCS2 Amp
pCAG.SARS-CoV-2-Myc-E pCAG.Myc Myc Amp
pCAG.SARS-CoV-2-E-Myc pCAG.MCS2-Myc Myc Amp
pcDNA3.1-sfGFP-LAMP1-mCherry pcDNA3.1 MycHis(−)A sfGFP,mCherry Amp
pEGFP-VPS4A K173Q pEGFP-C1 EGFP Kan
pQH-Gag pQC-xIN FLAG,HiBiT Amp
hACE2 Addgene#1786 Amp