FIG 1.

Env mutants used for this study. (A) Schematic representation of the truncation mutants used in this study with respect to structural elements within the CT. The mutants are named for the number of amino acids removed from the C-terminus of the CT. (B) The precise location of stop codons in the sequence of each truncation mutant is shown with the preceding LLP or other structural element; an asterisk indicates the position of the stop codon. (C) Depiction of the LLP2-3 region, which was selected for alanine scanning mutagenesis. Amino acids highlighted in red were found to be important for ERC localization of Env. (D) Structure of the HIV-1 Env transmembrane region coupled to the baseplate as proposed by Piai et al., image created using PyMol software using Mol* on the RCSB PDB site and PDB file 7LOH (19). The three trimer subunits are depicted in blue, gray, and magenta, while the CT of the gray subunit is further subdivided into red, orange, and yellow for LLP2, LLP3, and LLP1, respectively. (E) The location of amino acid residues highlighted in C mapped onto the structure in D.