FIG 2.
Colocalization of envelope with FIP1C560–649 requires LLP3 and the C-terminal segment of LLP2. (A) HeLa cells were transfected with WT or truncated Env and GFP-FIP1C560–649 for 48 h. Cells were then fixed, permeabilized, and stained with human anti-gp120 antibody 2G12, shown in red. GFP-FIP1C560–649 representing the condensed ERC is shown in green. Bar = 8 µm. (B) Colocalization of WT or truncated Env with GFP-FIP1C560–649 was quantified from 20 cells per Env mutant across more than three separate experiments; Manders colocalization coefficient is reported as mean ± SD. Significance was assessed using a one-way analysis of variance with Dunnett’s correction for multiple comparisons. ***P < 0.001.