Fig 2.
Detection of substrate specificity of NarA2B2 in E. coli BL21(DE3). (A) NAP (50 mg/L); (B) PHE (50 mg/L); (C) FLN (50 mg/L); (D) ACE (50 mg/L); (E) CA (50 mg/L); (F) DBT (50 mg/L); (G) PYE (10 mg/L); and (H) BP (50 mg/L). The yellow line with circles represents E. coli BL21(DE3) containing pETDuet-narA2-Ter-narB2 and pACYCDuet-phtAcAd, and the green line with squares represents E. coli BL21(DE3) containing pETDuet and pACYCDuet. The concentration in parentheses is the initial concentration. All the tests above were conducted in triplicate. NAP, naphthalene; PHE, phenanthrene; FLN, fluorene; ACE, acenaphthene; CA, carbazole; DBT, dibenzothiophene; PYE, pyrene; and BP, biphenyl.