Fig 7.

The purification and characterization of NarA2B2. (A) The purification of NarA2B2. (B) The purification of PhtAcAd. (C) The degradation of PHE by purified NarA2B2. NarA2B2: the reaction mixture contained purified NarA2B2 and PhtAcAd; CK: the reaction mixture did not contain purified NarA2B2 and PhtAcAd. (D) GC-MS profile of PHE degraded by purified NarA2B2. The sample at 0 h was used as the control, and the samples at 5 and 19 h were the experimental group (P1–P4: monohydroxy-PHE, TMS); (E) The catalytic temperature range suitable for NarA2B2 when PHE was used as the substrate; (F) The catalytic pH range suitable for NarA2B2 when PHE was used as the substrate.