TABLE 1.
Primers used for the construction of plasmids
| Primers | Sequences |
|---|---|
| 966-F1 | 5-CCACAGCCAGGATCCGATGGTCGTTCAAAATGGAATTGTA-3 |
| 966-R1 | 5-CGCCGAGCTCGAATTTTACTCGCTCATCAACTTAGCC-3 |
| 967-F | 5-GAAGGAGATATACATATGGGAGACGCGATCTATTT-3 |
| 967-R | CCAATTGAGATCTGCTTAGAAAAACACCCCGAGATTCT-3 |
| 966-H302A-F | 5-AACGCAGGCACCATCTTTCCCAACATGTC-3 |
| 966-H302A-R | 5-GCCTGCGTTATATGCCAGACGCCG-3 |
| 966-F216A-F | 5-AATGCAGTCGGGGATGCTTATCACACGTT-3 |
| 966-F216A-R | 5-GACTGCATTATCTCCGCCGAGCTT-3 |
| 966-T223A-F | 5-CACGCATTCATGACCCATTATTCTGCTGT-3 |
| 966-T223A-R | 5-GAATGCGTGATAAGCATCCCCGAC-3 |
| 966-D219A-F | 5-GGGGCAGCTTATCACACGTTCATGACCCA-3 |
| 966-D219A-R | 5-AGCTGCCCCGACAAAATTATCTCC-3 |
| 966-H222A-F | 5-TATGCAACGTTCATGACCCATTATTCTGC-3 |
| 966-H222A-R | 5-CGTTGCATAAGCATCCCCGACAAA-3 |
| 966-H227A-F | 5-ACCGCATATTCTGCTGTCCAGTTGAAGAT-3 |
| 966-H227A-R | 5-ATATGCGGTCATGAACGTGTGATA-3 |
| 966-F243A-F | 5-AAGGCAGCGTATTATGGGGTTCACGTATCGTGTGA-3 |
| 966-F243A-R | 5-CGCTGCCTTTGGATCGCCGCTCGG-3 |
| 966-Y300A-F | 5-GCAGCAAACCATGGCACCATCTTTCCCAACATGT-3 |
| 966-Y300A-R | 5-GTTTGCTGCCAGACGCCGGAACGC-3 |
| 966-W316A-F | 5-ACCGCAGCCACGGAAGATGGCAAG-3 |
| 966-W316A-R | 5-GGCTGCGGTGAAGTAACCGAACGACATGTT-3 |
| 966-F369A-F | 5-ACCGCAGGTGCGAGCGGTATGCTG-3 |
| 966-F369A-R | 5-ACCTGCGGTCCGAACAAACGTTTCTCTGGC-3 |
| 966-V236A-F | 5-ATCGCACCGAGCGGCGATCCA-3 |
| 966-V236A-R | 5-CGGTGCGATCTTCAACTGGAC-3 |
| 966-D379A-F | 5-GACGCAGCGGAAATGTGGAGCAACATCAC-3 |
| 966-D379A-R | 5-CGCTGCGTCCTGTTCCAGCATACC-3 |