Fig 8.

Rab5 and Rab7 are required for MSRV endocytosis. (A) The knockdown efficiency of Rab5. EPC cells were transfected with siCtrl, siRab5-1, or siRab5-2. At 24 h post transfection, the cells were collected for detecting the quantity of Rab5 by RT-qPCR. β-actin was used as the internal control. (B and C) Rab5 knockdown reduced the replication of MSRV. EPC cells were transfected with siRab5-2 or siCtrl, and infected with MSRV (MOI of 0.1) at 24 h post transfection. At 24 hpi, the viral RNA of infected cells was determined by RT-qPCR, and the N protein expression was detected by WB. (D) The knockdown efficiency of Rab7. The experiment procedure was the same as above. (E and F) Rab7 knockdown reduced the replication of MSRV. The experiment procedure was the same as above.