Fig 2.
Assessment of B/Malaysia/2506/2004 transmission between vaccinated guinea pigs in a 6-day contact transmission setting. Naïve donor guinea pigs were anaesthetized and challenged with 104 (A and B) or 105 (C and D) PFU of B/Malaysia/2506/2004. The following day, donor and vaccinated recipient (recipient 1) transmission pairs were co-caged (contact transmission). On day 6 post initial donor challenge, the recipient guinea pig (recipient 1) was removed and rehoused with another vaccinated recipient guinea pig (recipient 2). Recipient 2 was re-homed again on day 12 post initial donor challenge with vaccinated recipient 3. On days 2, 4, 6, 8, and 10 post contact, nasal washes were collected from anaesthetized donor (gray) and recipient (non-gray) guinea pigs. Arrows depict the addition of a recipient and the removal of a donor/recipient. The experiment was repeated three times with each replicate containing an unvaccinated donor and recipient 1, recipient 2, and recipient 3.