Fig 3.

Effects of N-linked glycans on SADS-CoV infection. Vero and Huh-7 cells were pretreated with 2000 U PNGase F for 3 h and then infected with SADS-CoV (MOI = 0.1). Viral replication in Vero cells treated with PNGase F was detected by IFA (A) and qRT-PCR (B). Viral replication in Huh7 cells treated with PNGase F was detected by IFA (C) and qRT-PCR (D). (E) Binding between SADS-CoV S1 and 100 N-glycans was detected using glycan microarrays. (F) Binding of SADS-CoV S1 with Neu5Gc and Neu5Ac was detected using glycan microarrays. All data are shown as means ± standard errors of the means (n = 3 biological replicates). Scale bars: 125 µm.