Fig 5.

Effects of other protease inhibitors on SADS-CoV infection. For the detection of viral replication dynamics, Vero and Huh-7 cells were pretreated with protease inhibitors (50 µM CA-074, 50 µM Z-FY-CHO, 500 nM BafA1, and 50 µM camostat) for 3 h and then infected with SADS-CoV (MOI = 0.1). The supernatants were harvested at 0, 24, or 48 hpi for the assessment of viral replication dynamics by qRT-PCR (A). Alternatively, cells were fixed at 24 hpi and stained with anti-SADS-CoV NP antibodies for detection by IFA (B). For viral entry detection, Vero and Huh-7 cells were pretreated with protease inhibitors for 3 h and then infected with SADS-CoV (MOI = 0.1) for 6 h. The cells were lysed, and virus detection was performed using qRT-PCR (C). Alternatively, cells were fixed and stained with anti-SADS-CoV NP antibodies for detection by IFA (D). All data are shown as means ± standard errors of the means (n = 3 biological replicates). Scale bars: 125 µm (B) and 300 µm (D).