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. 2023 Oct 27;16:867–883. doi: 10.2147/OTT.S429128

Table 1.

The Primer Sequences for the qPCR

Gene Sequence Amplicon Size TM (°C)
GAPDH F: CCATGGGGAAGGTGAAGGTC 113 bp 60.03
R: GAAGGGGTCATTGATGGCAAC 59.52
BAX F: TTTGCTTCAGGGTTTCATCC 162 bp 56.21
R: ATCCTCTGCAGCTCCATGTT 59.08
BCL-2 F: GAGGATTGTGGCCTTCTTTG 116 bp 56.69
R: GCCGGTTCAGGTACTCAGTC 60.11
Caspase-3 F: TGGAACAAATGGACCTGTTGACC 244 bp 61.25
R: AGGACTCAAATTCTGTTGCCACC 61.31
Caspase-9 F: AAGCCAACCCTAGAAAACATTACC 120 bp 59.23
R: GACATCACCAAATCCTCCAGAAC 59.31
Caspase-1 F: TACCTCTTCCCAGGACATTA 110 bp 54.52
R: ATGGACTTTCAGTACCCTTTC 55.21
Beclin-1 F: TGAAATCAATGCTGCCTGGG 162 bp 58.81
R: CCAGAACAGTATAACGGCAACTCC 61.45
XCT F: AAGGTGCCACTGTTCATCCC 198 bp 60.25
R: TGTTCTGGTTATTTTCTCCGACA 58.04
LC3A F: GACCGCTGTAAGGAGGTGC 153 bp 60.45
R: CTTGACCAACTCGCTCATGTTA 58.67
LC3B F: GATGTCCGACTTATTCGAGAGC 167 bp 58.7
R: TTGAGCTGTAAGCGCCTTCTA 59.45
P62 F: GCACCCCAATGTGATCTGC 92 bp 59.19
R: CGCTACACAAGTCGTAGTCTGG 60.73
FTH1 F: GGTGCGCCAGAACTACCAC 111 bp 61.03
R: TCGCGGTCAAAGTAGTAAGACATGG 62.84
E-Cadherin F: GTCTGTAGGAAGGCACAGCC 161 bp 60.39
R: TCATCCTCTGGGGGCAGTAA 59.96
N-Cadherin F: AGAGGCTTCTGGTGAAATCGC 214 bp 60.68
R: TGGAAAGCTTCTCACGGCAT 59.96