Fig 5.

Substrate spectrum of propane monooxygenase and molecular docking of PrmA and MTBE in the active site pocket and substrate tunnel. (A) Degradation of THF, 1,4-D, ETBE, TAME, TBA, and butane by E. coli Rosetta (DE3) heterologously expressing propane monooxygenase. All points represent the mean values of triplicate trials with error bars denoting the standard deviations. (B) Molecular docking of the receptor PrmA and ligand MTBE (CDOCKER energy = −13.26 kcal/mol). Two black boxes with dotted lines represent the binding pockets of the substrate tunnel and active site pocket of PrmA. Yellow dotted lines represent nonbonding interaction between residues and MTBE. Cyan-colored residues represent the amino acids that differ between strains JOB5 and PH-06. The orange spheres represent iron atoms, red sticks represent oxygen atoms, green sticks and cyan sticks represent carbon atoms, and blue sticks represent nitrogen atoms.