TABLE 2.
Primers used in this study
| Primers | Sequence (5′−3′)a | Purpose |
|---|---|---|
| pET28a-prmABCD-F | CTTTAAGAAGGAGATATACCATGTTCGCCCGCAGATCCGC | Design pET28a-prmABCD expression vector |
| pET28a-prmABCD-R | ATCTCAGTGGTGGTGGTGGTGGTGCGCGGATACCGGGGCCGACT | |
| pET28a-prmA-F | CTTTAAGAAGGAGATATACCATGTTCGCCCGCAGATCCGC | Design pET28a-prmA expression vector |
| pET28a-prmA-R | ATCTCAGTGGTGGTGGTGGTGGTGGATCTCGCTGAACGCGTCGG | |
| pET28a-prmB-F | CTTTAAGAAGGAGATATACCATGAGCGCCGAACTGATATC | Design pET28a-prmB expression vector |
| pET28a-prmB-R | ATCTCAGTGGTGGTGGTGGTGGTGCGACGTCACCGCCGCAGGAA | |
| pET28a-prmC-F | CTTTAAGAAGGAGATATACCATGAGCGACACCCAGTCCCA | Design pET28a-prmC expression vector |
| pET28a-prmC-R | ATCTCAGTGGTGGTGGTGGTGGTGGAACTTCGCCGGTTCGAGCTGA | |
| pET28a-prmD-F | CTTTAAGAAGGAGATATACCATGGCGCGCGTCACCCTGGT | Design pET28a-prmD expression vector |
| pET28a-prmD-R | ATCTCAGTGGTGGTGGTGGTGGTGCGCGGATACCGGGGCCGACT | |
| pET28a-prmAB-F | CTTTAAGAAGGAGATATACCATGTTCGCCCGCAGATCCGC | Design pET28a-prmAB expression vector |
| pET28a-prmAB-R | ATCTCAGTGGTGGTGGTGGTGGTGCGACGTCACCGCCGCAGGAA | |
| pET28a-prmABC-F | CTTTAAGAAGGAGATATACCATGTTCGCCCGCAGATCCGC | Design pET28a-prmABC expression vector |
| pET28a-prmABC-R | ATCTCAGTGGTGGTGGTGGTGGTGGAACTTCGCCGGTTCGAGCTGA | |
| pET28a-prmABD-F | CTGCGGCGGTGACGTCGTGAATGGCGCGCGTCACCCTGGT | Design pET28a-prmABD expression vector |
| pET28a-prmABD-R | ACCAGGGTGACGCGCGCCATTCACGACGTCACCGCCGCAG | |
| pET28a-prmBCD-F | CTTTAAGAAGGAGATATACCATGAGCGCCGAACTGATATC | Design pET28a-prmBCD expression vector |
| pET28a-prmBCD-R | ATCTCAGTGGTGGTGGTGGTGGTGCGCGGATACCGGGGCCGACT | |
| pET28a-prmA-FLAG-F | TGTCATGATCTTTATAATCACCGTCATGGTCTTTGTAGTCGATCTCGCTGAACGCGTCGG | Design pET28a-prmA-FLAG expression vector |
| pET28a-prmA-FLAG-R | TAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTGATGAGCGCCGAACTGATA | |
| pET28a-prmB-FLAG-F | TGTCATGATCTTTATAATCACCGTCATGGTCTTTGTAGTCCGACGTCACCGCCGCAGGAA | Design pET28a-prmB-FLAG expression vector |
| pET28a-prmB-FLAG-R | TAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTGAGCGACACCCAGTCCCAC | |
| pET28a-prmC-FLAG-F | TGTCATGATCTTTATAATCACCGTCATGGTCTTTGTAGTCGAACTTCGCCGGTTCGAGC | Design pET28a-prmC-FLAG expression vector |
| pET28a-prmC-FLAG-R | TAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTGATTGGAGGGCAGATGGCG | |
| pET28a-prmD-FLAG-F | TGTCATGATCTTTATAATCACCGTCATGGTCTTTGTAGTCCGCGGATACCGGGGCCGACT | Design pET28a-prmD-FLAG expression vector |
| pET28a-prmD-FLAG-R | TAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTGAGATCCGGCTGCTAACAA | |
| pET28a-prm-I84V-F | TTATGGAGGTCATGAAGCCG | Design pET28a-prm-I84V expression vector |
| pET28a-prm-I84V-R | CGGCTTCATGACCTCCATAA | |
| pET28a-prm-G95A-F | TTGGTCGACTTCGCCGAGTA | Design pET28a-prm-G95A expression vector |
| pET28a-prm-G95A-R | TACTCGGCGAAGTCGACCAA | |
| pET28a-prm-P165A-F | TCGAGCGGGGCGGGCGGTGTT | Design pET28a-prm-P165A expression vector |
| pET28a-prm-P165A-R | AACACCGCCCGCCCCGCTCGA | |
| pET28a-prm-A269S-F | ATTTCCAGGGCTCGGTTTAC | Design pET28a-prm-A269S expression vector |
| pET28a-prm-A269S-R | GTAAACCGAGCCCTGGAAAT | |
| pET28a-prm-V270T-F | GGGCGCGACTTACGACTACT | Design pET28a-prm-V270T expression vector |
| pET28a-prm-V270T-R | AGTAGTCGTAAGTCGCGCCC | |
| pET28a-prm-Q288E-F | TACTGGGACGAGTGGATCTG | Design pET28a-prm-Q288E expression vector |
| pET28a-prm-Q288E-R | CAGATCCACTCGTCCCAGTA | |
| pET28a-F | GAAACAAGCGCTCATGAG | Verify gene expression vector |
| pET28a-R | CCGGATATAGTTCCTCCT | |
| pJV53-prmABCD-F | TACGAGATCGGCGGCCGCATATGACTGCATCGGTCACCAC | Design pJV53-prmABCD expression vector |
| pJV53-prmABCD -R | GTCGGAATTCGCCGGGGCGCTCAGTGGTGGTGGTGGTGGTGCGCGGATACCGGGGCCGACT | |
| pJV53-F | GCCGCAGTTGTTCTCGCATA | Verify gene expression vector |
| pJV53-R | ACGTCAGGTGGCTAGCTGAT |
a
Underlined nucleic acid sequences represent homologous sequences derived from plasmids.