Fig. 6.

Overexpression of pax3a and sox10 induces ectopic expression of mitfa in zebrafish. (A-D′) mitfa expression at 6 hpf in control (A,A′), pax3a synthetic RNA-injected (B,B′), sox10 synthetic RNA injected (C,C′) and pax3a and sox10 synthetic RNA co-injected (D,D′) embryos. (A-D) Lateral views. (A′-D′) Animal pole views. mitfa mRNA was not expressed endogenously in 6 hpf embryo (A). Whereas overexpression of pax3a or sox10 by synthetic RNA injection into 1- to 2-cell-stage embryos failed to induce mitfa expression at 6 hpf, simultaneous overexpression of pax3a and sox10 can induce ectopic expression of mitfa. Scale bars: 200 µm. Fractions were 23/23 for pax3a injection, 16/16 for sox10 injection and 30/38 for pax3a and sox10 co-injection.