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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Mol Genet Metab. 2023 Jan 13;138(2):107508. doi: 10.1016/j.ymgme.2023.107508

Figure 5. Glycosphingolipid accumulation in Glb1−/− mice.

Figure 5.

Quantification of ganglioside levels in cortex, cerebellum and midbrain from Glb1+/+ and Glb1−/− mice. a. HPLC profile trace of cortex glycosphingolipids from a representative control at 32-weeks of age and Glb1−/− female mouse at 8-, 20-, 32- weeks of age. b. Cortex GA1 and GM1a levels. c. HPLC profile trace of cerebellum glycosphingolipids from a representative control at 32-weeks of age and Glb1−/− female mouse at 8-, 20-, 32- weeks of age. d. Cerebellum GA1 and GM1a levels. e. HPLC profile trace of midbrain glycosphingolipids from a representative control at 32-weeks of age and Glb1−/− female mouse at 8-, 20-, 32- weeks of age. f. Midbrain GA1 and GM1a levels. GSLs were extracted and digested with ceramide glycanase. Released oligosaccharides were 2-AA-labelled and analyzed using NP-HPLC. Data are presented as mean ± SD for n = 10 (5 males and 5 females) of 8-, 20- and 32-week-old mice (two-way ANOVA with Tukey multiple comparison test).