(A) The effect of PTNP on the viability of several cell lines. (B) The cell viability of HaCaT cells in 24, 48, and 72 hours of incubation with PTNP. (C) Cell cytotoxicity of HaCaT cells incubated with PTNP in different concentrations (0.1, 0.05, 0.1 and 0.25 mg/mL) and time points. Cells were irradiated at 808 nm (0.25, and 0.5 W/cm2). Statics are a 2-tailed t test and mixed-model ANCOVA with SAS 9.5 software from SAS Institute. *P < 0.05. The data are presented as mean ± SD. All graphs are representative of n = 6 experiments.