FIGURE 6.

(A) Images of the aortic valve with Oil Red O staining and the percentage area of lesions (Oil Red O positive, n=5–6). The mice were divided into 5 groups according to diet: a BD, amylin liver metabolic dysfunction-associated steatohepatitis (amylin MASH) (MASH group), amylin MASH with elobixibat alone (E) and cholestyramine alone (C), or amylin MASH with a combination of EC. (B) Images of the total aorta with Oil Red O staining and the percentage area of plaque (n=5–6). (C) Serum LDL and HDL cholesterol levels (n=5–7). (D) Images of the livers of diethylnitrosamine-treated mice fed a BD, amylin MASH (MASH group), amylin MASH with elobixibat alone (E) and cholestyramine alone (C), or amylin MASH with a combination of EC. Tumor count, tumor count (>5 mm in diameter), and average log₂ tumor burden (n=9–10). Data are presented as the mean±SE. Significance was determined using Student t test. *p<0.05, **p<0.01. Abbreviations: BD, basal diet; C, cholestyramine; E, elobixibat; EC, elobixibat and cholestyramine; MASH, metabolic dysfunction-associated steatohepatitis.