Figure S4.

Measuring changes in viral RNA synthesis with SLAM-seq, related to Figure 4

(A) Mismatch frequencies observed in SLAM-seq libraries.

(B) Principal component analysis of SLAM-seq experiments.

(C) Analysis of SLAM-seq experiments at 12 hpi using GRAND-SLAM. Scatter plot of average log₂ fold changes in total RNA (x axis) and newly synthesized RNA (y axis) is shown for SND1 knockout (KO) and control (CTRL) cells, relative to wild-type (WT) cells (n = 2 independent experiments). Insets show a zoom-in view on viral genes for improved visibility.

(D) Bar graph visualization of log₂ fold changes for new RNA (left) and total RNA (right) observed in SLAM-seq experiments shown in (C) and Figure 4C for viral genes at 6 (top) and 12 hpi (bottom).