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. 2023 Oct 31;6:1104. doi: 10.1038/s42003-023-05481-y

Fig. 1. Long-read nanopore RNA sequencing to identify full-length transcripts in VSMCs.

Fig. 1

a Schematic of experimental design for this study. Primary HASMCs (passage 3 to 6) were transfected with negative control miRNA or hsa-miR-221-3P (50 nM) for 36 h. Subsequently, cells were treated with vehicle control, PDGF (15 ng/mL), or TGFβ (10 ng/ml) for another 24 h and analyzed. n = 4 for each group. b Composition of different transcript types in all detected full-length transcripts. c Proportion of genes with annotated and unannotated transcripts in different gene types. d Stack plot shows the fractions of genes detected in different sequencing technologies in each sample. e Bar plot shows the number of transcripts in genes detected in ONT platform. f Length distribution of annotated and unannotated transcripts detected in ONT platform.