Table 2.
Cryo-EM data collection and model refinement details
| Parameters | 70S ribosome | 50S ribosomal subunit |
|---|---|---|
| Cryo-EM | ||
| Electron microscope | Titan Krios G3 | Titan Krios G3 |
| Voltage (kV) | 300 | 300 |
| Pixel size (Å) | 1.0961 | 1.0961 |
| Defocus range (µM) | 0.8–2.5 | 0.8–2.5 |
| Electron dose/image | 67.5 | 67.5 |
| Particles | 288,776 | 12,449 |
| Software | cryoSPARC v2.15 | cryoSPARC v2.15 |
| Symmetry | C1 | C1 |
| FSC threshold | 0.143 | 0.143 |
| Average B-factor (Å2) | 57.2 | 96.6 |
| Resolution (Å) | 2.9 | 3.4 |
| Atomic model | ||
| R.M.S. deviations | ||
| Bond lengths (Å) | 0.000 | 0.000 |
| Bond angles (°) | 0.089 | 0.103 |
| Resolved protein residues | 6239 | 3569 |
| Protein rotamer outliers | 22 (0.4%) | 13 (0.4%) |
| Ramachandran favored | 5831 (93.5 %) | 3280 (93.0 %) |
| Ramachandran outliers | 1 (0.016%) | 1 (0.028%) |
| Resolved RNA residues | 4646 | 2867 |
| RNA sugar pucker outliers | 64 (1.4%) | 33 (1.2%) |
| RNA suite outliers | 790 (17.0%) | 428 (14.9%) |
| RNA Suiteness | 0.61 | 0.64 |
| Clash score | 7.5 | 7.1 |