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. 2023 Oct 31;13:18736. doi: 10.1038/s41598-023-45470-z

Figure 2.

Figure 2

Overexpression of apelin plasmids and colocalization with S100A4 in SMCs. (A) Expression of mutated apelin/eGFP constructs is observed by live imaging of SMCs: Scale bar = 10 μm. Representative confocal microscopy staining (B) and nuclear quantification (C) of apelin and S100A4 expression in S-SMCs 96 h after transfection with S.Ap (n = 23 nuclei) or N. Ap (n = 29 nuclei)-encoded plasmids. Nuclei are stained in blue with DAPI. Scale bar = 10 μm. (D) 2.5 × magnified region highlighted by the dashed square in (B) and showing apelin and S100A4 expression in the nucleus. Scale bar = 4 μm.