Fig. 2. C1qA was the key recognition unit of the complement system related to Rituximab resistance and might be regulated by m⁶A modification.

A The mRNA levels of 3 subunits of C1q in Farage/S and Farage/R cells were detected by qPCR. B The levels of C1qA, C1qB, and C1qC proteins were detected by western blotting. C C1qA expression was localized by immunofluorescence. D The expressions of C1qA in DLBCL tissues from the first surgery of three pairs of rituximab sensitive and rituximab-resistant patients were showed by IHC assay. E A schematic diagram of the C1qA promoter inserted into the luciferase expression vector. F The relative expression levels of 2 transcriptional regulators, GATA-1 and MafB, were detected by qPCR. G The ratios of luciferase activity in the Farage/S and Farage/R groups. H The level of total m⁶A-modified RNA. I The level of m⁶A-modified C1qA mRNA as detected by Me-RIP qPCR. ns, not significant; ***p < 0.001.