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. 2023 Oct 2;55(10):2190–2204. doi: 10.1038/s12276-023-01089-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The Bone-mPACT+ method involved the use of 20% EDTA, Calci-Clear Rapid, 5% nitric acid, and 10% formic acid. The method was further optimized for processing rodent bones, which require a long clearing time for tissue processing. The Bone-mPACT+ Advance method was optimized for bone marrow and protected it from swelling. Bone tissue sections larger than a mouse bone could be prepared in a 4% acrylamide-based hydrogel coating.