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. 2023 Oct 2;55(10):2190–2204. doi: 10.1038/s12276-023-01089-8

Fig. 5. Generation of transparent mouse tibia samples by passive bone clearing methods.

Fig. 5

a Schematic representation and timeline of the passive bone clearing methods. The individual reagents or processes used in the fourteen clearing methods for the decalcification and clearing process are shown. b Comparison of optical transparency achieved in mouse tibia samples processed using Bone-mPACT+ (with 20% EDTA), Bone-CLARITY, CUBIC-L/R, MACS, Ce3D, Fast 3D Clear, EZ Clear, PEGASOS, BABB, methanol BABB, 3DISCO, uDISCO, FDISCO, and BoneClear. The transparency of all the cleared samples was evident when they were placed on a patterned background (length × width = 5 × 5 mm).