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. 2023 Oct 2;55(10):2190–2204. doi: 10.1038/s12276-023-01089-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a OPG (green) and DAPI (blue) immunostaining in ICR mouse femur samples processed using Bone-mPACT+ plus 20% EDTA. (left) The upper (z = 166–235 μm) and lower (z = 456–784 μm) images of the mouse femur samples were 3 × 11 tiled. A 3D projection of OPG (green) expression, focusing on the distal epiphysis (3 × 3 tiled, range: 200 μm) and femoral head (2 × 2 tiled, range: 360 μm), including the bone marrow (1 × 1 tiled, range: 180 μm). b RUNX2 (green) and DAPI (blue) immunostaining in ICR mouse femur samples processed using Bone-mPACT+ plus 20% EDTA. Whole images (3 × 11 tiled, range: 133–676 μm) of RUNX2 (green) and DAPI (blue) expression in mouse femur bone. A 3D projection of RUNX2 (green) expression focusing on the distal epiphysis (3 × 4 tiled, range: 527 μm) and femoral head (2 × 2 tiled, range: 243 μm), including the bone marrow in the upper (2 × 2 tiled, range: 237 μm) and lower (1 × 1 tiled, range: 208 μm) images. All images were acquired with 10× and 40× objectives on a confocal laser microscope. Scale bar (white: 500 μm, yellow: 100 μm, magenta: 10 μm).