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. 2023 Oct 31;13:18763. doi: 10.1038/s41598-023-45976-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Biological functions and mechanistic studies of the prognostic biomarker. (a) Expression level of CNFN in tumor and normal tissues detected using RT-qPCR; (b) Expression level of CNFN in tumor tissues between lymph node metastasis and non-lymph node metastasis detected using RT-qPCR; (c–e) Cell proliferation capacity was validated using colony formation and CCK-8 assays; f: Cell migration ability was evaluated using wound healing assay; (g) Transwell migration assay was used to assess the effects of CNFN on cell migration ability; (h) Transwell invasion assay was utilized to evaluate the effects of CNFN on cell invasion ability; (i) Western blotting was used to detect changes in the expression levels of EMT-associated markers after silencing of CNFN.