Figure 3.

p53 mediates quiescence through PPARα

(A) RNA-seq log₂ expression ratios of Ppara and Ppard targets in indicated cultures.

(B) RNA-seq log₂ expression ratios of FAO regulators in indicated cultures.

(C) qRT-PCR of Ppargc1a mRNA levels in p53^+/+ and p53^−/−NPCs alone and in co-culture. n = 4.

(D and E) p53 CUT&RUN read coverage at Ppargc1a (D) and Cdkn1a (E) gene loci for alone and co-cultured NPCs.

(F) Quantification of EdU FACS profiles of indicated cultures treated or untreated with PPARα agonist WY-14643 or omega-3 PUFAs (EPA/DHA). Fold change. n = 4.

(G and H) FA-driven oxygen consumption in p53^+/+ (G) and p53^−/− (H) NPC co-cultures treated or untreated with WY-14643. n = 7.

(I) OCR of the signal profiles shown in (G) and (H). n = 7.

(J) ¹³C enrichment in co-cultured p53^+/+ and p53^−/− NPCs treated or untreated with WY-14643. n = 4.

All graphs mean ± SEM except in (A) where boxplots represent median and interquartile range; two-sided Wilcoxon test (A), two-way ANOVA with Tukey’s multiple-comparisons test (C, F, I, and J). p values: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

See also Figure S3.