REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-Ki67	Abcam	Cat# ab16667; RRID: AB_302459
Mouse monoclonal anti-Ascl1 (Clone 24B72D11.1)	kindly gifted by F. Guillemot (Francis Crick Institute, UK). Urbán et al.47	N/A
Mouse monoclonal anti-p53	Cell Signaling	Cat# 2524; RRID_331743
Rabbit polyclonal anti-GFAP	Agilent	Cat# Z0344; RRID: AB_10013382
Mouse monoclonal anti-Sox2	Abcam	Cat# ab79351;RRID: AB_10710406
Mouse monoclonal anti-Nestin	Santa Cruz	Cat# sc-33677; RRID: AB_627995
Monoclonal anti-mCD24 PE (Clone M1/69)	BD Pharmingen	Cat# 12-0242-82; RRID: AB_465602
Monoclonal anti-mCD133 Biotin (Clone 13A4)	eBioscience	Cat# 13-1331-82; RRID: AB_466591
PE-Cy7 conjugated streptavidin	eBioscience	Cat# 25-4317-82; RRID: AB_10116480
Rabbit polyclonal anti-RFP	Antibodies Online	Cat# ABIN129578 RRID: AB_10781500
Monoclonal anti-mCD24 eFluor (Clone M1/69)	eBioscience	Cat# 48-0242-82; RRID: AB_1311169
Monoclonal anti-PPAR alpha	Arigo Biolaboratories	Cat# ARG55240; N/A
Bacterial and Virus Strains
Ad-CMV-iCre	Vector Biolabs	Cat# 1045
Ad-CMV-Null	Vector Biolabs	Cat# 1300
Chemicals, Peptides, and Recombinant Proteins
EGF-complexed to Alexa Fluor™ 647	ThermoFisher	Cat# E35351
Tamoxifen	Sigma	Cat# T5648
EdU (5-ethynyl-2′-deoxyuridine)	Santa Cruz	Cat# sc-284628
Fish oil	Sigma	Cat# F8020
PPARa agonist WY-14643	Sigma	Cat# C7081
Etomoxir	Sigma	Cat# E1905
Palmitic acid	Sigma	Cat# P5585
BSA Conjugated Docosahexaenoic Acid (DHA, 22:6n-3)	Cloud-Clone Corp	Cat# CPO122Ge11
BSA-conjugated Eicosapentaenoic Acid (EPA, 20:5n-3)	Cloud-Clone Corp	Cat# CPO632Ge11
Critical Commercial Assays
Zombie Green™ Fixable Viability Kit	BioLegend	Cat# 423111
Click-iT™ EdU Alexa Fluor™ 647 Imaging kit	Life Technologies	Cat# C10340
Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry assay	Life Technologies	Cat# C10419
Fatty Acid Oxidation Assay kit	Abcam	Cat# ab217602
Extracellular Oxygen Consumption Assay kit	Abcam	Cat# ab197243
iScript gDNA clear cDNA synthesis kit	Bio-rad	Cat# 1725034
Deposited Data
RNA-seq data	This paper	GEO: GSE165801
CUT&RUN data	This paper	GEO: GSE165802
Codega et al RNA-seq dataset	Codega et al.9	GEO: GSE54653
Llorens-Bobadilla et al RNA-seq dataset	Llorens-Bobadilla et al.10	GEO: GSE67833
Mizrak et al RNA-seq dataset	Mizrak et al.11	GEO: GSE109447
Experimental Models: Cell Lines
Mouse: Primary mouse NPCs isolated at P9–P12	This paper	N/A
Mouse: Primary mouse brain microvascular endothelial cells	Cell Biologics	Cat# C57-6023
Experimental Models: Organisms/Strains
Mouse: FVB/N-Tg(GFAPGFP)14Mes/J	The Jackson Laboratory	Cat# Jax003257; RRID: IMSR_JAX:003257
Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J	The Jackson Laboratory	Cat# 007914 RRID: IMSR_JAX:007914
Mouse: p53LoxP/LoxP	Marino et al.48	N/A
Mouse: GLAST::CreERT	Mori et al.49	N/A
Mouse: C57Bl/6J	Charles River	RRID: IMSR_JAX:000664
Oligonucleotides
	See Table S2 for primer sequences	N/A
Software and Algorithms
Fiji ImageJ	Schindelin et al.50	https://imagej.net/Fiji
FlowJo version 10.6.2	Becton, Dickinson and Company	https://www.flowjo.com/
TopHat v.2.0.11	Kim et al.51	https://ccb.jhu.edu/software/tophat/index.shtml
DESeq2 Bioconductor package	Love et al.52	http://bioconductor.org/packages/release/bioc/html/DESeq2.html
HTSeq v.0.6.1	Anders et al.53	http://htseq.readthedocs.io/en/release_0.9.1/overview.html
Trimmomatic v.036	Bolger et al.54	http://www.usadellab.org/cms/index.php?page=trimmomatic
Bowtie2 v2.3.4.3	Langmead and Salzberg55	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Chippeakanno R package v3.20.1	Zhu et al.56	https://www.bioconductor.org/packages/release/bioc/html/ChIPpeakAnno.html
VLAD	Richardson and Bult57	http://proto.informatics.jax.org/prototypes/vlad/
MACS2 v2.1.1	Zhang et al.58	https://hbctraining.github.io/Intro-to-ChIPseq/lessons/05_peak_calling_macs.html
g:Profiler package	Raudvere et al.59	https://biit.cs.ut.ee/gprofiler/gost