Supplementary Figure S2.

Characteristics of T cells in CSCC and CAde. (a) Heatmap presented the expression profiles of markers genes in each identified T cell subcluster. (b) Heatmap displayed the identified GO enrichment terms of each identified T cell subcluster based on GSVA analysis. Color reflected the z-score. (c) Bar plot displayed the proportion of each subcluster in individual samples. (d) Kaplan–Meier curves regarding the overall survival based on the gene signature of cytotoxicity in CSCC cohort (left) and CAde cohort (right) from the TCGA cohort. The P-values were calculated by Log-rank test. (e–f) Developmental trajectory of CD8⁺ T cells constructed by monocle 2, colored by pseudo-time and pathologic types, respectively. (g–h) The cytotoxic and exhausted scores of CAde and CSCC changed dynamically with pseudo-time, respectively. The solid lines represented LOESS fitting of the relationship between estimated scores and Monocle component 1. P-value was calculated by t-test. (i) Dynamic changes of representative marker genes during the pseudo-time in CD8⁺ T cells.