Supplementary Figure S3.
Characteristics of myeloid cells in CSCC and CAde. (a) Violin plots displayed the gene expression profiles of selected marker genes in each identified subcluster. (b) Bar plot displayed the proportion of each medullary subcluster in individual samples. (c) Box plot depicted the composition comparison for each subcluster in CSCC and CAde. The P-values were calculated by Wilcoxon Rank Sum Test. (d) Tissue preference of each macrophage subcluster revealed by Ro/e (ratio of observed cell number to expected cell number). (e) Violin plots displayed the expression profiles of M1 and M2 gene signature in each identified macrophage subcluster. (f–h) Developmental trajectory of macrophage cells constructed by monocle 2, colored by pseudo-time (left), state (right) and cell subtype split by pathologic type. (i) Pseudo-time heatmap displayed the various genes involved in the developmental process of macrophages (left), and the enrichment GO terms of gene sets of different states were also displayed (right). (j) Scatter plots depicted the expression levels of some representative genes during pseudo-time. The fit curves represented the gene expression of two branches, and pathologic types were denoted by color.